» start page / index

 » ECP 2007

 » Cytology 2008

 » IAP seminars

 » University of Helsinki
    weekly seminars

 » Tampere University
    Hospital seminars

 » various seminars

 » search

Mucin Immunohistochemistry Laboratory
Jacques Bara
U-482 INSERM

Laboratoire d´Histologie
Nicole Maurin
CHU St-Antoine rue du Faubourg St Antoine, 75012 Paris, France
http://ifr31w3.toulouse.inserm.fr/mucines/
1. ABERRANT CRYPT FOCI (ACF) OF HUMAN COLON MUCOSA

 
Slide 1  
Slide 2  
Case 1
ACF of “typical” type according to Fenoglio’s classification [Toxi- cologic Pathology 27: 632-642 (1999)]. 82 years old patient, tissue is fixed with 95% ethanol during 24h and embedded in paraffin. Mucosa adjacent to an adenocarcinoma of right colon with an ACF.

Slide 1 = hematein/eosin staining
Slide 2 = immunoperoxidase staining using a mixture of anti-M1/MUC5AC Mabs [Tumor Biol: 24:109-115 (2003)].

Comments: note that the immunostaining is restricted to the goblet cells in the upper part of the crypts in ACF. The mucosa adjacent to the ACF is not stained by these antibodies.

  
 
Slide 3  
Slide 4  
Case 2
ACF of “hyperplastic” type according to Fenoglio’s classi- fication [Toxicologic Pathology 27: 632-642 (1999)]. 63 years old patient, tissue is fixed with 95% ethanol during 24h and embedded in paraffin. Mucosa adjacent to an adenocarcinoma of rectum with an ACF.

Slide 3 = hematein/eosin staining
Slide 4 = immunoperoxidase staining using a mixture of anti-M1/MUC5AC Mabs [Tumor Biol: 24:109-115 (2003)].

Comments: note that the immunostaining is restricted to the goblet cells located to the upper part of the crypts in ACF. The mucosa adjacent to the ACF is not stained by these antibodies.

  
 
Slide 5  
Slide 6  
Case 3
ACF of “hyperplastic” type according to Fenoglio’s classification [Toxicologic Pathology 27: 632-642 (1999)]. 61 years old patient, tissue is fixed with 95% ethanol during 24h and embedded in paraffin. Mucosa adjacent to an adenocarcinoma of rectum with an ACF.

Slide 5 = hematein/eosin staining
Slide 6 = immunoperoxidase staining using a mixture of anti-M1/MUC5AC Mabs [Tumor Biol: 24:109-115 (2003)].

Comments: note that the staining is restricted to the goblet cells located throughout the crypts of ACF. The mucosa adjacent to the ACF is not stained by these antibodies
  

2. MUCOSA ADJACENT TO COLON ADENOCARCINOMAS

 
Slide 7  
Slide 8  
Case 4
“Swiss roll” of the mucosa adjacent to a rectal adenocarcinoma of a 69 years old patient. A small fragment of the adenocarcinoma is visible in the center of the Swiss roll.

Slide 7 = hematein/eosin staining
Slide 8 = immunoperoxidase staining using a mixture of anti-M1/MUC5AC Mabs.

Comments: note the strong immunoreactivity of the transitional mucosa (the 30 crypts nearest of the adenocarcinoma) which is thicker owing to the elongation of crypts, certain crypts being branched. In some areas, the mucus struck to the surface of the mucosa is strongly positive. [Cancer Res: 44: 4040-4065 (1984)].

  

More information: http://ifr31w3.toulouse.inserm.fr/mucines/
J. Bara, M.E. Forgue-Lafitte and M.P. Buisine. Expression of Gastric MUC5AC mucin during colon
carcinogenesis. In Handbook of Immunochemistry and in situ Hybridization of human carcinoma,
Volume 2: Molecular Pathology, Colorectal Carcinomas and Prostate Carcinoma (6) 167-182(2004)
by M.A. Hayat, Kean University, Union, NI, USA in press


All material on this website is protected by copyright. By accessing or downloading contents
from this website, you expressly agree and consent to our terms and conditions.