Interactive Gleason grading
This is a new tool for learning and standardizing Gleason grading of prostate biopsies. A journal article describing the web site has been published in the Human Pathology journal. This web site also serves as a tool for assessing observer variability of Gleason grading, and as such, it was utilized in the study design: An experienced uropathologist scored a series of 62 biopsies, first conventionally using original glass slides, and 6 weeks later from virtual slides on the web site. The intraobserver agreement was excellent. The virtual slides were also viewed by 2 other uropathologists, with resulting interobserver coefficients within the range of previously reported studies using glass slides only.
Synchronized virtual slides
This is an example of viewing two stains made sequentially on a single slide. With this technique we are able to view and compare stains which never co-existed in real life. As an example, we show H&E staining and IHC of AMACR/p63 in 23 prostate needle biopsies (the latter done after de-staining H&E). Comparison of H&E and IHC is important in clinical diagnostics, but is difficult by conventional means. This site also has importance in prostate histopathology education. From a technical perspective, this web site demonstrates how virtual slides easily can be aligned for synchronized viewing. A journal article describing this technique has been published.
Medical Parasitology
The aim of this project is to adapt virtual microscopy for parasitology specimens. The project was motivated by difficulties in education and quality assurance both in Sweden and in Nicaragua. Virtual microscopy addresses major problems, such as specimen handling, availability, reproducibility, distribution and interpretation.
Automated quantification of fluorescence in situ hybridization signals
Using a popular, open source image manipulation tool, ImageJ, a macro for FISH signal assessment has been created. Combined with digitization of FISH samples, the FishJ macro enables gene copy number to be assessed and re-evaluated on any area of a digitized specimen.

Quality assurance
There are several ongoing QA projects, most of which require logins and passwords. QA projects
can be arranged on a cost-recovery basis. Please contact us for further information.

ERSPC prostate Gleason grading (password required)

For QA projects co-arranged with Labquality, please visit their website:

Labquality is a non-profit organization owned by hospitals and associations. External Quality Assessment
Programmes help laboratories to confirm the quality of results and indicate their competence.