Interactive Gleason grading
This is a new tool for learning and
grading of prostate
describing the web site has been published
Pathology journal. This web site also
serves as a tool for
variability of Gleason grading, and as
it was utilized
in the study design: An experienced uropathologist scored a series of 62
biopsies, first conventionally using original glass slides, and
6 weeks later from
virtual slides on
the web site. The intraobserver agreement
The virtual slides were
viewed by 2
the range of previously
using glass slides only.
Synchronized virtual slides
This is an example of viewing two stains made sequentially on a single slide.
With this technique we are able to view and
compare stains which never
co-existed in real life. As an example, we
show H&E staining and IHC of
AMACR/p63 in 23 prostate needle biopsies
(the latter done after de-staining
Comparison of H&E and IHC is
important in clinical diagnostics, but is
difficult by conventional means.
This site also has importance in prostate
histopathology education. From
a technical perspective, this web site
demonstrates how virtual slides
easily can be aligned for synchronized
journal article describing this
technique has been published.
The aim of this project is to adapt virtual microscopy for parasitology specimens. The project was motivated by difficulties in education and quality assurance both in Sweden and in Nicaragua. Virtual microscopy addresses major problems, such as specimen handling, availability, reproducibility, distribution and interpretation.
Automated quantification of fluorescence in situ hybridization signals
Using a popular, open source image manipulation tool, ImageJ, a macro for FISH signal assessment has been created.
Combined with digitization of FISH samples, the FishJ macro enables gene copy number to be assessed and re-evaluated on any area of a digitized specimen.
several ongoing QA projects, most of which
and passwords. QA projects
can be arranged
on a cost-recovery basis.
Please contact us for further information.
ERSPC prostate Gleason grading
For QA projects co-arranged with Labquality, please visit their
Labquality is a non-profit organization owned by hospitals and
Programmes help laboratories to confirm the quality
of results and
indicate their competence.